Molecular architecture of the "stressosome," a signal integration and transduction hub

被引：127

作者：

Marles-Wright, Jon ^{[2
]}

Grant, Tim ^{[1
]}

Delumeau, Olivier ^{[2
]}

van Duinen, Gijs ^{[1
]}

Firbank, Susan J. ^{[2
]}

Lewis, Peter J. ^{[3
]}

Murray, James W. ^{[2
]}

Newman, Joseph A. ^{[2
]}

Quin, Maureen B. ^{[2
]}

Race, Paul R. ^{[2
]}

Rohou, Alexis ^{[1
]}

Tichelaar, Willem ^{[1
]}

van Heel, Marin ^{[1
]}

Lewis, Richard J. ^{[2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Nat Sci, Div Mol Biosci, London SW7 2AZ, England

[2] Univ Newcastle, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[3] Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia

来源：

SCIENCE | 2008年 / 322卷 / 5898期

基金：

英国生物技术与生命科学研究理事会; 澳大利亚研究理事会;

关键词：

D O I：

10.1126/science.1159572

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress- responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium- resolution cryo- electron microscopy reconstruction of the 1.8- megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid- like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult.

引用

页码：92 / 96

页数：5

共 30 条

[1] New family of regulators in the environmental signaling pathway which activates the general stress transcription factor σB of Bacillus subtilis [J].