Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity

被引：111

作者：

Borges, Anabela ^{[1
,2
]}

Serra, Sofia ^{[3
,4
]}

Abreu, Ana Cristina ^{[1
]}

Saavedra, Maria J. ^{[2
]}

Salgado, Antonio ^{[3
,4
]}

Simoes, Manuel ^{[1
]}

机构：

[1] Univ Porto, Dept Chem Engn, Fac Engn, LEPABE, P-4100 Oporto, Portugal

[2] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, CECAV Vet & Anim Sci Res Ctr, Vila Real, Portugal

[3] Univ Minho, Life & Hlth Sci Res Inst ICVS, Sch Hlth Sci, Braga, Portugal

[4] ICVS 3Bs PT Govt Associate Lab, Braga, Portugal

来源：

BIOFOULING | 2014年 / 30卷 / 02期

关键词：

biofilms; Chromobacterium violaceum; cytotoxicity; isothiocyanates; phenolics; phytochemicals; quorum sensing inhibition; ENZYMATIC-HYDROLYSIS PRODUCTS; CHROMOBACTERIUM-VIOLACEUM; PSEUDOMONAS-AERUGINOSA; ANTIMICROBIAL ACTIVITY; ALLYL ISOTHIOCYANATE; MEDICINAL-PLANTS; PHENOLIC-ACIDS; ANTIBIOTIC-RESISTANCE; BIOFILM PREVENTION; BACTERIAL BIOFILMS;

D O I：

10.1080/08927014.2013.852542

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.

引用

页码：183 / 195

页数：13

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