The appearance and possible role of plasminogen activator of urokinase type (u-PA) activity in the cornea related to soft contact lens wear in rabbits

This is the first study in which u-PA activity is detected in situ during SCL wear. The histochemical localization of u-PA activity is done by the methods of Lojda using unfixed cryostat sections on semipermeable membranes and a gel incubation medium containing sensitive substrates with the 7-amino-4-trifluoromethylcoumarin (AFC) (Enzyme Systems Products, Sierra Lane, Dublin, CA, USA) leaving groups. Z-Gly-GIy-Arg-AFC and Glut-Gly-Arg-AFC were employed as the substrates. The results show that in the normal cornea u-PA activity is absent. Also the wearing of SCL does not evoke the appearance of u-PA activity in the cornea within the first three days. On day 4 the first u-PA activity appears; it is located in the superficial layers of the corneal epithelium. On day 7 of SCL wear, u-PA activity is present in all layers of the corneal epithelium and (to a lesser extent) also in the comeal endothelium; keratocytes of the corneal stroma are only slightly active for u-PA. Extended SCL wear (for two weeks) leads to an increase of u-PA activity in keratocytes beneath the epithelium. Also, some inflammatory cells (mainly polymorphonuclear leukocytes, PMNs) present in the corneal stroma are enzymatically active. After three weeks of SCL wear the number of PMNs in the corneal stroma increases; some PMNs are highly active for u-PA. In the corneal endothelium the u-PA activity is also highly pronounced. It can be concluded that extended SCL wear leads to the gradual increase of u-PA activity in the rabbit cornea. It is suggested that active u-PA is involved in the corneal damage related to SCL wear.