Small Vessel Replacement by Human Umbilical Arteries With Polyelectrolyte Film-Treated Arteries In Vivo Behavior

被引：46

作者：

Kerdjoudj, Halima

Berthelemy, Nicolas

Rinckenbach, Simon ^{[4
]}

Kearney-Schwartz, Anna ^{[2
]}

Montagne, Karine ^{[5
]}

Schaaf, Pierre ^{[6
]}

Lacolley, Patrick ^{[3
]}

Stoltz, Jean-Francois

Voegel, Jean-Claude ^{[7
]}

Menu, Patrick ^{[1
]}

机构：

[1] Nancy Univ, Grp Bioengn, Sch Med, LEMTA UMR CNRS 7563, F-54505 Vandoeuvre Les Nancy, France

[2] Nancy Univ, INSERM, U684, Ctr Invest Clin Nancy CIC P 9501, F-54505 Vandoeuvre Les Nancy, France

[3] Nancy Univ, INSERM, U684, Sch Med, F-54505 Vandoeuvre Les Nancy, France

[4] ULP Strasbourg, ERT 1061, Strasbourg, France

[5] Hop Brabois Adultes, Dept Pathol, Vandoeuvre Les Nancy, France

[6] CNRS, Inst Charles Sadron, UPR 22, Strasbourg, France

[7] Univ Strasbourg, INSERM, U595, Fac Odontol, Strasbourg, France

来源：

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY | 2008年 / 52卷 / 19期

关键词：

vascular tissue engineering; polyelectrolyte multilayer film; human umbilical arteries;

D O I：

10.1016/j.jacc.2008.08.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective The aim of this study was to evaluate the patency of human umbilical arteries treated with polyelectrolyte multilayers (PEMs) after rabbit implantation. Background The development of small-caliber vascular substitutes with high patency after implantation remains a real challenge for vascular tissue engineering. Methods Cryopreserved human umbilical arteries were enzymatically de-endothelialized and the luminal surfaces were coated with poly(styrene sulfonate)/poly(allylamine hydrochloride) (PSS/PAH) multilayers. The PEM-untreated arteries and PEM-treated rabbit carotids were used as graft control. The native rabbit carotids were bypassed by grafts. Results The Doppler ultrasound evaluation, performed in vivo, showed that all PEM-treated grafts remained patent during the full experimental period, whereas after only 1 week, no blood circulation was detected in untreated arteries. Scanning electron microscopy and histological graft examination showed pervasive thrombus formation on the luminal surface of untreated arteries after 1 week and clean luminal surface for treated arteries for at least up to 12 weeks. The arterial wall cells were identified through alpha-smooth muscle actin alpha nu delta platelet endothelial cell adhesion molecule-1 expression. The smooth muscle cells positive to alpha-smooth muscle actin were identified in adventitia and media and the endothelial cells positive to platelet endothelial cell adhesion molecule in intima. Von Kossa reaction didn't reveal any calcium salt deposits on the wall arteries, suggesting a good wall remodelling with no sign of graft rejection. Conclusions The in vivo evaluation of human umbilical arteries treated with PSS/PAH multilayers demonstrated a high graft patency after 3 months of implantation. Such modified arteries could constitute a useful option for small vascular replacement. (J Am Coll Cardiol 2008;52:1589-97) (C) 2008 by the American College of Cardiology Foundation

引用

页码：1589 / 1597

页数：9

共 43 条

[1]

Aldenhoff YBJ, 2001, J BIOMED MATER RES, V54, P224, DOI 10.1002/1097-4636(200102)54:2<224::AID-JBM9>3.0.CO

[2]

2-E

[3] Investigation of 3-D mechanical properties of blood vessels using a new in vitro tests system:: Results on sheep common carotid arteries [J].