In vitro blood compatibility of polymeric biomaterials through covalent immobilization of an amidine derivative

被引：38

作者：

Gouzy, MF

Sperling, C

Salchert, K

Pompe, T

Streller, U

Uhlmann, P

Rauwolf, C

Simon, F

Böhme, F

Voit, B

Werner, C

机构：

[1] Inst Polymer Res Dresden, D-01069 Dresden, Germany

[2] Max Bergmann Ctr Biomat, D-01069 Dresden, Germany

[3] Univ Toronto, Dept Mech & Ind Engn, Toronto, ON, Canada

来源：

BIOMATERIALS | 2004年 / 25卷 / 17期

关键词：

maleic anhydride copolymer; surface coating; thrombin inhibitor; immobilization; blood compatibility;

D O I：

10.1016/j.biomaterials.2003.10.091

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We present a surface coating with anticoagulant characteristics showing significantly reduced coagulation activation. The synthesis of a monomeric conjugate containing a benzamidine moiety was carried out and its inhibitory activity against human thrombin, the key enzyme of the blood coagulation cascade, was determined using a chromogenic assay. Based on that, low-thrombogenic interfaces were prepared by covalent attachment of this low-molecular weight thrombin inhibitor on poly(octadecene-alt-maleic anhydride) copolymer thin films and characterized using ellipsometry, XPS and dynamic contact angle measurements. The in vitro hemocompatibility tests using freshly drawn human whole blood showed, in agreement with the SEM images, that a PO-MA film modified with a benzamidine moiety using a PEG spacer decreased the activation of coagulation, platelets and the complement system. The decreased protein adsorption, in addition to the specific inhibition of thrombin, effectively enhanced the short-term hemocompatibility characteristics. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：3493 / 3501

页数：9

共 37 条

[1] Immobilization of a nonsteroidal antiinflammatory drug onto commercial segmented polyurethane surface to improve haemocompatibility properties [J].