Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid

被引：23

作者：

Bartra, SS ^{[1
]}

Jackson, MW ^{[1
]}

Ross, JA ^{[1
]}

Plano, GV ^{[1
]}

机构：

[1] Univ Miami, Miller Sch Med, Dept Microbiol & Immunol, Miami, FL 33101 USA

来源：

INFECTION AND IMMUNITY | 2006年 / 74卷 / 02期

关键词：

D O I：

10.1128/IAI.74.2.1381-1386.2006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.

引用

页码：1381 / 1386

页数：6

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