Protective effect of quercetin on lead-induced oxidative stress and endoplasmic reticulum stress in rat liver via the IRE1/JNK and PI3K/Akt pathway

被引:128
作者
Liu, C. -M. [1 ]
Zheng, G. H. [1 ]
Ming, Q. L. [1 ]
Sun, J. M. [1 ]
Cheng, C. [1 ]
机构
[1] Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plant Jiangsu Prov, Tangshan New Area, Xuzhou 221116, Jiangsu, Peoples R China
关键词
quercetin; lead; ER stress; Akt; JNK; liver; INDUCED APOPTOSIS; ACTIVATION; ANTIOXIDANT; EXPRESSION; CADMIUM; NEPHROTOXICITY; INVOLVEMENT; PROGRESSION; CHAPERONES; TOXICITY;
D O I
10.3109/10715762.2012.760198
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lead (Pb), a well-known environmental toxin, is one of the major hazards for human health. Quercetin (QE), a natural flavonoid, has been reported to have many benefits and medicinal properties. However, its protective effects against Pb-induced endoplasmic reticulum (ER) stress in liver have not been clarified. The aim of the present study was to investigate the effects of quercetin on hepatic ER stress in rats exposed to Pb. Wistar rats were exposed to lead acetate in the drinking water with or without quercetin co-administration for 75 days. Our data showed that quercetin significantly prevented Pb-induced hepatotoxicity in a dose-dependent manner, indicated by both diagnostic indicators of liver damage and histopathological analysis. Quercetin markedly decreased Pb contents in blood and liver. Western blot analysis showed that Pb-induced ER stress in rat liver was significantly inhibited by quercetin. In exploring the underlying mechanisms of quercetin action, we found quercetin markedly suppressed Pb-induced oxidative stress. Quercetin decreased reactive oxygen species (ROS) production and increased the total antioxidant capacity in rat livers. Additionally, quercetin dramatically increased Phosphoinositide-3-kinase (PI3K) and phosphorylated protein kinase B (PKB/Akt) levels in liver rats. In the examined unfolded protein response (UPR) pathways, quercetin markedly inhibited the Pb-induced increase of the phosphorylated inositol-requiring enzyme 1 (IRE1) and c-jun N-terminal kinase (JNK) in rat liver. Taken together, these results suggested that the inhibition of Pb-induced ER stress by quercetin is due at least in part to its anti-oxidant stress activity and its ability to modulate the PI3K/Akt and IRE1/JNK signaling pathway.
引用
收藏
页码:192 / 201
页数:10
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