Isolation, purification and characterization of GPI-anchored membrane proteins from Trypanosoma rangeli and Trypanosoma cruzi

被引：9

作者：

Añez-Rojas, N ^{[1
]}

机构：

[1] Univ Los Andes, Fac Ciencias, Dept Biol, Merida 5101, Venezuela

来源：

ACTA TROPICA | 2006年 / 97卷 / 02期

关键词：

GPI; membrane proteins; Trypanosoma cruzi; Trypanosoma rangeli; species differentiation;

D O I：

10.1016/j.actatropica.2005.09.005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学]; 100103 [病原生物学];

摘要：

GPI-anchored proteins from plasma membrane of Trypanosoma rangeli and Trypanosoma cruzi epimastigotes were isolated and characterized using the partition Triton X-114 method. The detection by Western blot of specific proteins of 90, 85 and 56 kDa molecular mass in T. rangeli compared to those of 30, 70 and 100 kDa detected in T. cruzi demonstrates specific discrimination between these two species of Trypanosoma. The potential diagnostic value of the here reported proteins to differentiate mixed infections by T. cruzi and T. rangeli is evaluated and its potential for epidemiological studies of Chagas disease in endemic areas is also discussed. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：140 / 145

页数：6

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