The two analogous phosphoglycerate mutases of Escherichia coli

被引：90

作者：

Fraser, HI ^{[1
]}

Kvaratskhelia, M ^{[1
]}

White, MF ^{[1
]}

机构：

[1] Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland

来源：

FEBS LETTERS | 1999年 / 455卷 / 03期

关键词：

glycolysis; phosphoglycerate mutase; vanadate inhibition; cofactor requirement; Escherichia coli;

D O I：

10.1016/S0014-5793(99)00910-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glycolytic enzyme phosphoglycerate mutase exists in two evolutionarily unrelated forms. Vertebrates have only the 2,3-bisphosphoglycerate-dependent enzyme (dPGM), whilst higher plants have only the cofactor-independent enzyme (iPGM). Certain eubacteria possess genes encoding both enzymes, and their respective metabolic roles and activities are unclear. We have over-expressed, purified and characterised the two PGMs of Escherichia coli, Both are expressed at high levels, but dPGM has a 10-fold higher specific activity than iPGM. Differential inhibition by vanadate was observed, The presence of an integral manganese ion in iPGM was confirmed by EPR spectroscopy. (C) 1999 Federation of European Biochemical Societies.

引用

页码：344 / 348

页数：5

共 16 条

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