The 5′ region of the COX4 gene contains a novel overlapping gene, NOC4

被引:17
作者
Bachman, NJ
Wu, W
Schmidt, TR
Grossman, LI
Lomax, MI
机构
[1] Univ Michigan, Dept Anat & Cell Biol, Ann Arbor, MI 48109 USA
[2] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI 48201 USA
关键词
D O I
10.1007/s003359901031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We identified a novel human gene, NOC4 (Neighbor Of COX4). located 5' to COX4, the gene for cytochrome c oxidase subunit IV, on Chr16q32-ter. Transcripts from this gene were identified among human expressed sequence tags. A full-length, 1.06-kb human retinal NOC4 cDNA encoded a 24-kDa, 210-amino acid hypothetical protein of unknown function. Northern hybridization analysis of human RNAs from various tissues detected NOC4 transcripts of 2.2 and 1.4 kb in all tissues examined, suggesting that NOC4 expression is ubiquitous. Transcription of both the COX4 and NOC4 genes initiates within a 250-bp intergenic promoter and occurs in opposite directions. The bidirectional promoter is G + C-rich, lacks TATA and CCAAT elements, and contains multiple potential binding sites for Spl and NRF-2/GABP. Two of the NRF-2/GABP sites are located within 14-bp direct repeats, a conserved feature of mammalian COX4 promoters. The NOC4 and COX4 genes are also linked in the rat, mouse, and bovine genomes. A NOC4-GFP fusion protein is located in both the nucleus and the cytoplasm, including the mitochondria.
引用
收藏
页码:506 / 512
页数:7
相关论文
共 52 条
[1]  
ADAMS MD, 1995, NATURE, V377, P3
[2]   COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].
ADAMS, MD ;
KELLEY, JM ;
GOCAYNE, JD ;
DUBNICK, M ;
POLYMEROPOULOS, MH ;
XIAO, H ;
MERRIL, CR ;
WU, A ;
OLDE, B ;
MORENO, RF ;
KERLAVAGE, AR ;
MCCOMBIE, WR ;
VENTER, JC .
SCIENCE, 1991, 252 (5013) :1651-1656
[3]   Cell respiration is controlled by ATP, an allosteric inhibitor of cytochrome-c oxidase [J].
Arnold, S ;
Kadenbach, B .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 249 (01) :350-354
[4]  
Ausubel F., 1990, CURRENT PROTOCOLS MO
[5]   ISOLATION AND CHARACTERIZATION OF THE FUNCTIONAL GENE ENCODING BOVINE CYTOCHROME-C-OXIDASE SUBUNIT-IV [J].
BACHMAN, NJ .
GENE, 1995, 162 (02) :313-318
[6]   SUBCELLULAR LOCATIONS OF MOD5 PROTEINS - MAPPING OF SEQUENCES SUFFICIENT FOR TARGETING TO MITOCHONDRIA AND DEMONSTRATION THAT MITOCHONDRIAL AND NUCLEAR ISOFORMS COMMINGLE IN THE CYTOSOL [J].
BOGUTA, M ;
HUNTER, LA ;
SHEN, WC ;
GILLMAN, EC ;
MARTIN, NC ;
HOPPER, AK .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) :2298-2306
[7]   STRUCTURE AND FUNCTION OF CYTOCHROME-C-OXIDASE [J].
CAPALDI, RA .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :569-596
[8]  
CARTER RS, 1992, J BIOL CHEM, V267, P23418
[9]   CLONING AND CHARACTERIZATION OF THE MOUSE CYTOCHROME-C-OXIDASE SUBUNIT-IV GENE [J].
CARTER, RS ;
AVADHANI, NG .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (01) :97-106
[10]  
CARTER RS, 1994, J BIOL CHEM, V269, P4381