A metagenome-wide association study of gut microbiota in type 2 diabetes

被引：5248

作者：

Qin, Junjie ^{[1
]}

Li, Yingrui ^{[1
]}

Cai, Zhiming ^{[2
]}

Li, Shenghui ^{[1
]}

Zhu, Jianfeng ^{[1
]}

Zhang, Fan ^{[3
]}

Liang, Suisha ^{[1
]}

Zhang, Wenwei ^{[1
]}

Guan, Yuanlin ^{[1
]}

Shen, Dongqian ^{[1
]}

Peng, Yangqing ^{[1
]}

Zhang, Dongya ^{[1
]}

Jie, Zhuye ^{[1
]}

Wu, Wenxian ^{[1
]}

Qin, Youwen ^{[1
]}

Xue, Wenbin ^{[1
]}

Li, Junhua ^{[1
]}

Han, Lingchuan ^{[3
]}

Lu, Donghui ^{[3
]}

Wu, Peixian ^{[3
]}

Dai, Yali ^{[3
]}

Sun, Xiaojuan ^{[3
]}

Li, Zesong ^{[2
]}

Tang, Aifa ^{[2
]}

Zhong, Shilong ^{[2
]}

Li, Xiaoping ^{[4
]}

Chen, Weineng ^{[1
]}

Xu, Ran ^{[1
]}

Wang, Mingbang ^{[1
]}

Feng, Qiang ^{[1
]}

Gong, Meihua ^{[1
]}

Yu, Jing ^{[1
]}

Zhang, Yanyan ^{[1
]}

Zhang, Ming ^{[1
]}

Hansen, Torben ^{[5
]}

Sanchez, Gaston ^{[6
,7
]}

Raes, Jeroen ^{[8
,9
]}

Falony, Gwen ^{[8
,9
]}

Okuda, Shujiro ^{[8
,9
]}

Almeida, Mathieu ^{[10
]}

LeChatelier, Emmanuelle ^{[10
]}

Renault, Pierre ^{[10
]}

Pons, Nicolas ^{[10
]}

Batto, Jean-Michel ^{[10
]}

Zhang, Zhaoxi ^{[1
]}

Chen, Hua ^{[1
]}

Yang, Ruifu ^{[1
,11
]}

Zheng, Weimou ^{[1
]}

Li, Songgang ^{[1
]}

Yang, Huanming ^{[1
]}

机构：

[1] BGI Shenzhen, Shenzhen 518083, Peoples R China

[2] Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China

[3] Peking Univ, Shenzhen Hosp, Shenzhen 518036, Peoples R China

[4] Guangdong Acad Med Sci, Guangdong Gen Hosp, Med Res Ctr, Guangzhou 510080, Peoples R China

[5] Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Basic Metab Res, DK-2100 Copenhagen, Denmark

[6] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94820 USA

[7] Univ Calif Berkeley, Dept Stat, Berkeley, CA 94820 USA

[8] VIB, Dept Biol Struct, B-1050 Brussels, Belgium

[9] Vrije Univ Brussel, Dept Appl Biol Sci DBIT, B-1050 Brussels, Belgium

[10] INRA, F-78350 Jouy En Josas, France

[11] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[12] Univ Copenhagen, Inst Biomed Sci, DK-8000 Aarhus, Denmark

[13] Univ Aarhus, Fac Hlth Sci, DK-8000 Aarhus, Denmark

[14] Hagedorn Res Inst, DK-2820 Gentofte, Denmark

[15] Hagedorn Res Inst, DK-2820 Gentofte, Denmark

[16] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark

来源：

NATURE | 2012年 / 490卷 / 7418期

基金：

中国国家自然科学基金;

关键词：

OBESITY; DIVERSITY; BACTEREMIA; INFECTION; STRESS;

D O I：

10.1038/nature11450

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes.

引用

页码：55 / 60

页数：6

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