Electrically induced NGF production by astroglial cells

被引：45

作者：

Koyama, S

Haruyama, T

Kobatake, E

Aizawa, M

机构：

[1] Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku

来源：

NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 02期

关键词：

astroglial cells; nerve growth factor; electric stimulation; protein kinase C;

D O I：

10.1038/nbt0297-164

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A controlled culture system has been developed to induce nerve growth factor (NGF) production in astroglial cells that are cultured on an electrode surface. The electrode potential is alternatively modulated at an amplitude of 300 mV and a frequency of 10 Hz. The electric stimulation triggers NGF production and secretion. The mechanism of the electrically induced NGF production is discussed in association with protein kinase C (PKC) activation.

引用

收藏

页码：164 / 166

页数：3

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