Closing and sequencing of the 5' region of the human glucose-6-phosphatase gene: Transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

被引：77

作者：

Schmoll, D ^{[1
]}

Allan, BB ^{[1
]}

Burchell, A ^{[1
]}

机构：

[1] UNIV DUNDEE,NINEWELLS HOSP & MED SCH,DEPT OBSTET & GYNAECOL,DUNDEE DD1 9SY,SCOTLAND

来源：

FEBS LETTERS | 1996年 / 383卷 / 1-2期

关键词：

glucose-6-phosphatase; dexamethasone; insulin; gene regulation; cAMP;

D O I：

10.1016/0014-5793(96)00224-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced the first 1.2 kb of the 5' region of the human glucose-6-phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity, Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.

引用

页码：63 / 66

页数：4

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