Characterization of hybrid poly(acriflavine)/FAD films and their electrocatalytic properties with NAD+ and NADH

Hybrid films composed of electropolymerized acriflavine-flavin adenine dinucleotide (AF/FAD) film modified electrodes have been prepared in neutral aqueous solution. These films were found to be stable and electrochemically active on glassy carbon, gold, and transparent semiconductor tin oxide electrodes. The hybrid poly (acriflavine)/FAD film showed two redox couples. Electrochemical quartz crystal microbalance and cyclic voltammetry were used to study the in situ growth of hybrid poly (acriflavine)/FAD films. Electrocatalytic oxidation and reduction of dihydronicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide ion (NAD(+)) by a poly (acriflavine)/FAD hybrid film in aqueous solution were carried out. The poly (acriflavine)/FAD hybrid films were found active for both electrocatalytic oxidation as well as reduction reaction of NADH. The electrocatalytic reduction of L-cystine, S4O62-, SO52-, S2O82-, NO2-, and H2O2 by a poly (acriflavine)/FAD hybrid film in a neutral aqueous solution was found to be an electrocatalytic reduction activity. (c) 2006 The Electrochemical Society.