Validation of a single nucleotide polymorphism genotyping assay for the human serum paraoxonase gene using electronically active customized microarrays

被引：7

作者：

Nagan, N ^{[1
]}

O'Kane, DJ ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Div Clin Biochem & Immunol, Rochester, MN 55905 USA

来源：

CLINICAL BIOCHEMISTRY | 2001年 / 34卷 / 07期

关键词：

paraoxonase; microarrays; genotyping; single nucleotide polymorphisms (SNP);

D O I：

10.1016/S0009-9120(01)00266-1

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: Develop a microarray based genotyping assay to detect SNPs in human paraoxonase (PON I) and compare its accuracy with DNA sequencing and RFLP based assays. Design and Methods: Amplicons spanning the polymorphic regions of interest were genotyped by sequencing, RFLP, and microarray technology. Validation parameters included precision, linearity of signal response, and carryover between adjacent sites on the microarray. Results: A 100% correlation in results obtained using DNA sequencing, RFLP and microarray technology was observed. Conclusions: The microarray technology provides an accurate and reliable assay platform with applicability in high throughput genotyping studies. (C) 2001 The Canadian Society of Clinical Chemists. All rights reserved.

引用

页码：589 / 592

页数：4

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