Use of the modified robbins device and fluorescent staining to screen plant extracts for the inhibition of S-mutans biofilm formation

被引：34

作者：

Honraet, K ^{[1
]}

Nelis, HJ ^{[1
]}

机构：

[1] Univ Ghent, Lab Pharmaceut Microbiol, B-9000 Ghent, Belgium

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 64卷 / 02期

关键词：

S; mutans; MRD; SYTO (R) 9; biofilm; hydroxyapatite;

D O I：

10.1016/j.mimet.2005.05.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Streptococcus mutans plays an important role in the formation of dental plaque. To study biofilm growth on hydroxyapatite (HA) in vitro, a flow system based on a Modified Robbins Device (MRD) and a method for the quantification of the biomass using fluorescent staining with SYTO (R) 9 were developed. The combined approach was used to assess the inhibitory effect of plant extracts on biofilm formation in concentrations below their minimal inhibitory concentrations. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：217 / 224

页数：8

共 43 条

[1]

Inefficient delivery of yeast cells as an explanation for reduced plating efficiency of Candida albicans [J].