Transcriptional profiling of mouse and human ES. cells identifies SLAIN1, a novel stem cell gene

被引：43

作者：

Hirst, CE

Ng, ES

Azzola, L

Voss, AK

Thomas, T

Stanley, EG

Elefanty, AG ^{[1
]}

机构：

[1] Monash Univ, Monash Immunol & Stem Cell Labs, Clayton, Vic 3800, Australia

[2] Walter & Eliza Hall Inst Med Res, Parkville, Vic 3050, Australia

来源：

DEVELOPMENTAL BIOLOGY | 2006年 / 293卷 / 01期

基金：

英国医学研究理事会;

关键词：

embryonic stem cell; cell differentiation; transcriptional profiling; SLAIN1;

D O I：

10.1016/j.ydbio.2006.01.023

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We analyzed the transcriptional profiles of differentiating mouse embryonic stem cells (mESCs) and show that embryoid bodies (EBs) sequentially expressed genes associated with the epiblast, primitive streak, mesoderm and endoderm of the developing embryo, validating ESCs as a model system for identifying cohorts of genes marking specific stages of embryogenesis. By comparing the transcriptional profiles of undifferentiated ESCs to those of their differentiated progeny, we identified 503 mESC and 983 hESC genes selectively expressed in undifferentiated ES cells. Over 75% of the mESC genes were expressed in hESC and vice versa, attesting to the underlying similarity of mESCs and hESCs. The expression of a cohort of 68 genes decreased greater than 2-fold during differentiation in both mESCs and hESCs. As well as containing many validated ESC genes such as Oct4 [Pou5fl], Nanog and Nodal, this cohort included an uncharacterised gene (FLJ30046), which we designated SLAIN1/Slain1. Slain1 was expressed at the stem cell and epiblast stages of ESC differentiation and in the epiblast, nervous system, tailbud and somites of the developing mouse embryo. SLAIN1 and its more widely expressed homologue SLAIN2 comprise a new family of structurally unique genes conserved throughout vertebrate evolution. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：90 / 103

页数：14

共 65 条

[1] Unique gene expression signatures of independently-derived human embryonic stem cell lines [J].

Abeyta, MJ ;

Clark, AT ;

Rodriguez, RT ;

Bodnar, MS ;

Pera, RAR ;

Firpo, MT .

HUMAN MOLECULAR GENETICS, 2004, 13 (06) :601-608

[2] Standardizing global gene expression analysis between laboratories and across platforms [J].

Bammler, T ;

Beyer, RP ;

Bhattacharya, S ;

Boorman, GA ;

Boyles, A ;

Bradford, BU ;

Bumgarner, RE ;

Bushel, PR ;

Chaturvedi, K ;

Choi, D ;

Cunningham, ML ;

Dengs, S ;

Dressman, HK ;

Fannin, RD ;

Farun, FM ;

Freedman, JH ;

Fry, RC ;

Harper, A ;

Humble, MC ;

Hurban, P ;

Kavanagh, TJ ;

Kaufmann, WK ;

Kerr, KF ;

Jing, L ;

Lapidus, JA ;

Lasarev, MR ;

Li, J ;

Li, YJ ;

Lobenhofer, EK ;

Lu, X ;

Malek, RL ;

Milton, S ;

Nagalla, SR ;

O'Malley, JP ;

Palmer, VS ;

Pattee, P ;

Paules, RS ;

Perou, CM ;

Phillips, K ;

Qin, LX ;

Qiu, Y ;

Quigley, SD ;

Rodland, M ;

Rusyn, I ;

Samson, LD ;

Schwartz, DA ;

Shi, Y ;

Shin, JL ;

Sieber, SO ;

Slifer, S .

NATURE METHODS, 2005, 2 (05) :351-356

[3]

Barnett LD, 2000, METH MOL B, V158, P65

[4] Gene expression in human embryonic stem cell lines: unique molecular signature [J].

Bhattacharya, B ;

Miura, T ;

Brandenberger, R ;

Mejido, J ;

Luo, YQ ;

Yang, AX ;

Joshi, BH ;

Ginis, I ;

Thies, RS ;

Amit, M ;

Lyons, I ;

Condie, BG ;

Itskovitz-Eldor, J ;

Rao, MS ;

Puri, RK .

BLOOD, 2004, 103 (08) :2956-2964

[5] Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei [J].

Bortvin, A ;

Eggan, K ;

Skaletsky, H ;

Akutsu, H ;

Berry, DL ;

Yanagimachi, R ;

Page, DC ;

Jaenisch, R .

DEVELOPMENT, 2003, 130 (08) :1673-1680

[6] Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation [J].

Brandenberger, R ;

Wei, H ;

Zhang, S ;

Lei, S ;

Murage, J ;

Fisk, GJ ;

Li, Y ;

Xu, CH ;

Fang, R ;

Guegler, K ;

Rao, MS ;

Mandalam, R ;

Lebkowski, J ;

Stanton, LW .

NATURE BIOTECHNOLOGY, 2004, 22 (06) :707-716

[7]

Brandenberger Ralph, 2004, BMC Developmental Biology, V4, P1

[8] Signalling, cell cycle and pluripotency in embryonic stem cells [J].

Burdon, T ;

Smith, A ;

Savatier, P .

TRENDS IN CELL BIOLOGY, 2002, 12 (09) :432-438

[9]

BURDSAL CA, 1993, DEVELOPMENT, V118, P829

[10] Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b [J].

Chen, TP ;

Ueda, Y ;

Dodge, JE ;

Wang, ZJ ;

Li, E .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (16) :5594-5605

← 1 2 3 4 5 6 7 →