Crystallization and preliminary X-ray diffraction analysis of the interleukin-3 alpha receptor bound to the Fab fragment of antibody CSL362

被引：8

作者：

Broughton, Sophie E. ^{[1
,2
]}

Hercus, Timothy R. ^{[3
]}

Nero, Tracy L. ^{[1
,2
]}

Dhagat, Urmi ^{[1
,2
]}

Owczarek, Catherine M.

Hardy, Matthew P. ^{[4
]}

Fabri, Louis J. ^{[4
]}

Scotney, Pierre D. ^{[4
]}

Nash, Andrew D. ^{[4
]}

Wilson, Nicholas J. ^{[4
]}

Lopez, Angel F. ^{[3
]}

Parker, Michael W. ^{[1
,2
,5
]}

机构：

[1] St Vincents Inst Med Res, Australian Canc Res Fdn Rat Drug Discovery, Fitzroy, Vic 3065, Australia

[2] St Vincents Inst Med Res, Biota Struct Biol Lab, Fitzroy, Vic 3065, Australia

[3] SA Pathol, Ctr Canc Biol, Div Human Immunol, Adelaide, SA, Australia

[4] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, CSL Ltd, Parkville, Vic 3052, Australia

[5] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3052, Australia

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2014年 / 70卷

基金：

英国医学研究理事会;

关键词：

ACUTE MYELOGENOUS LEUKEMIA; STRUCTURAL BASIS; COMPLEX REVEALS; STEM-CELLS; IL-3; CHAIN; ACTIVATION; SOFTWARE; SYSTEM; MODE;

D O I：

10.1107/S2053230X14002593

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Interleukin-3 (IL-3) is a member of the beta common family of cytokines that regulate multiple functions of myeloid cells. The IL-3 receptor-specific alpha subunit (IL3R alpha) is overexpressed on stem cells/progenitor cells of patients with acute myeloid leukaemia, where elevated receptor expression correlates clinically with a reduced patient survival rate. The monoclonal antibody (MAb) CSL362 is a humanized MAb derived from the murine MAb 7G3, originally identified for its ability to specifically recognize the human IL-3 receptor and for blocking the signalling of IL-3 in myeloid and endothelial cells. In order to elucidate the molecular mechanism of CSL362 antagonism, a preliminary structure of human IL3R alpha in complex with the MAb CSL362 has been determined.

引用

页码：358 / 361

页数：4

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