A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes

被引：38

作者：

Decker, H ^{[1
]}

Gaisser, S ^{[1
]}

Pelzer, S ^{[1
]}

Schneider, P ^{[1
]}

Westrich, L ^{[1
]}

Wohlleben, W ^{[1
]}

Bechthold, A ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST PHARMAZEUT,D-72076 TUBINGEN,GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 141卷 / 2-3期

关键词：

Actinomyces; Streptomyces; polymerase chain reaction; 6-deoxyhexose; genetic screening of dNDP-glucose dehydratases;

D O I：

10.1111/j.1574-6968.1996.tb08384.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Oligonucleotide primers were designed and successfully applied to amplify DNA fragments of dNDP-glucose dehydratase genes from actinomycete species producing natural compounds which contain deoxysugar moieties. The deduced amino acid sequence of the isolated fragments revealed similarity to known dNDP-glucose dehydratases. A phylogeny for the deduced proteins of the obtained fragments and for dNDP-glucose dehydratases described in the data bases was constructed. dNDP-glucose dehydratases from actinomycetes were more related to each other than to dehydratases from species of other orders. The phylogenetic analysis also revealed a close relation between dehydratases from strains producing natural compounds with similar deoxysugar moieties.

引用

页码：195 / 201

页数：7

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