Progress and Prospects: The design and production of plasmid vectors

被引：133

作者：

Gill, D. R. ^{[1
]}

Pringle, I. A. ^{[1
]}

Hyde, S. C. ^{[1
]}

机构：

[1] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Oxford OX3 9DU, England

来源：

GENE THERAPY | 2009年 / 16卷 / 02期

关键词：

non-viral; plasmid; minicircle; promoter; transgene expression; SLEEPING-BEAUTY TRANSPOSITION; EPISOMAL TRANSGENE EXPRESSION; RNA INTERFERENCE; DNA METHYLATION; GENE-EXPRESSION; HISTONE MODIFICATIONS; NUCLEAR IMPORT; IN-VIVO; DELIVERY; PROMOTER;

D O I：

10.1038/gt.2008.183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminating CG dinucleotides (CpGs) from the pDNA are also considered.

引用

页码：165 / 171

页数：7

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