Fatty alcohols production by oleaginous yeast

被引：83

作者：

Fillet, Sandy ^{[1
]}

Gibert, Jordi ^{[1
]}

Suarez, Beatriz ^{[1
]}

Lara, Armando ^{[1
]}

Ronchel, Carmen ^{[1
]}

Adrio, Jose L. ^{[1
]}

机构：

[1] Neol Biosolut SA, Granada 18016, Spain

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2015年 / 42卷 / 11期

关键词：

Fatty alcohols; Metabolic engineering; Fatty acyl-CoA reductase; Rhodosporidium toruloides; ACYL-COA REDUCTASE; SACCHAROMYCES-CEREVISIAE; ENHANCED PRODUCTION; BIOSYNTHESIS; STORAGE; LIPIDS; OIL; METABOLISM; COENZYME; PATHWAY;

D O I：

10.1007/s10295-015-1674-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C-16-C-18 fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date.

引用

收藏

页码：1463 / 1472

页数：10

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