Inhibition of oncogene expression using vector-generated RNA antisense

被引：7

作者：

Whitesell, L

Liu, X

Bagatell, R

机构：

[1] Univ Arizona, Steele Mem Childrens Res Ctr, Tucson, AZ 85724 USA

[2] Univ Arizona, Hlth Sci Ctr, Dept Pediat, Tucson, AZ 85724 USA

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1999年 / 18卷 / 03期

关键词：

D O I：

10.1006/meth.1999.0787

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antisense RNA expression vectors have been developed relatively recently as a means to study the role of specific oncogenes in malignant transformation. In this paper, strategies for the construction of antisense plasmid vectors from commercially available reagents are described. Techniques for the introduction of these vectors into cell lines and tumors are also described and preferred methods for the evaluation of biological effects are presented. Lastly, using specific examples, the limitations and potential artifacts associated with antisense vector use in the study of tumorigenesis are discussed. (C) 1999 Academic Press.

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页码：296 / 303

页数：8

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