Design of division specific primers of Ralstonia solanacearum and application to the identification of European isolates

被引：33

作者：

Boudazin, G

Le Roux, AC

Josi, K

Labarre, P

Jouan, B

机构：

[1] INRA, Lab Pathol Vegetale, F-49071 Beaucouze, France

[2] INRA, FNPPPT, Lab Pathol Vegetale, F-35653 Le Rheu, France

[3] Lab Nacl Protect Vegetaux, F-49000 Angers, France

[4] INRA, Lab Pathol Vegetale, F-35653 Le Rheu, France

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 1999年 / 105卷 / 04期

关键词：

16S rRNA gene; diagnosis; PCR; Ralstonia solanacearum;

D O I：

10.1023/A:1008763111230

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A PCR diagnostic test for detection of Ralstonia solanacearum at the infraspecific level was developed, based on polymorphisms within the 16S rRNA gene sequence. Partial sequences of this gene were determined for three French isolates which showed the characteristics of R. solanacearum subdivision 2a described by Taghavi et al. (1996). Oligonucleotide primers were designed to incorporate the nucleotide triplet (at positions 458-460 of the 16S rRNA gene) which differs between divisions 1 and 2 16S rRNA sequences of R. solanacearum isolates. A simple PCR test unambiguously revealed the division of each isolate. The PCR test was useful for identification of isolates of R. solanacearum from Europe.

引用

页码：373 / 380

页数：8

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