Automation of functional assays by flow injection fluorescence microscopy

被引：11

作者：

Scampavia, LD ^{[1
]}

Hodder, PS ^{[1
]}

Lähdesmäki, I ^{[1
]}

Ruzicka, J ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 11期

关键词：

D O I：

10.1016/S0167-7799(99)01375-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bead-injection spectroscopy is a novel technique that uses immobilized eukaryotic cells on microbeads as a renewable biosensor for fluorescence microscopy. The use of a flow injection instrument allows fast functional assays that generate full kinetic characterization of a drug. Because the cell population is automatically replaced for each assay, Variability is minimized, thus allowing greater accuracy.

引用

页码：443 / 447

页数：5

共 20 条

[1] USING MICROPHYSIOMETRY TO STUDY THE PHARMACOLOGY OF EXOGENOUSLY EXPRESSED M(1) AND M(3) MUSCARINIC RECEPTORS [J].