Rab13 regulates PKA signaling during tight junction assembly

被引：76

作者：

Köhler, K ^{[1
]}

Louvard, D ^{[1
]}

Zahraoui, A ^{[1
]}

机构：

[1] CNRS, Lab Morphogenesis & Cell Signaling, UMR 144, Inst Curie, F-75248 Paris 05, France

来源：

JOURNAL OF CELL BIOLOGY | 2004年 / 165卷 / 02期

关键词：

Rab GTPase; phosphorylation; VASP; cell-cell junctions; epithelial cells;

D O I：

10.1083/jcb.200312118

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs) through a yet unknown mechanism. Here, we show that expression of the GTP-bound form of Rab13 inhibits PKA-dependent phosphorylation and TJ recruitment of the vasodilator-stimulated phosphoprotein, an actin remodelling protein. We demonstrate that Rab13GTP directly binds to PKA and inhibits its activity. Interestingly, activation of PKA abrogates the inhibitory effect of Rab13 on the recruitment of vasodilator-stimulated phosphoprotein, ZO-1, and claudin1 to cell-cell junctions. Rab13 is, therefore, the first GTPase that controls PKA activity and provides an unexpected link between PKA signaling and the dynamics of TJ assembly.

引用

页码：175 / 180

页数：6

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