Rapid Detection of Salmonella in Foods Using Real-Time PCR

被引：95

作者：

Cheng, Chorng-Ming ^{[1
]}

Lin, Wen ^{[1
]}

Van, Khanh Thien ^{[1
]}

Phan, Lieuchi ^{[1
]}

Tran, Nelly N. ^{[1
]}

Farmer, Doris ^{[2
]}

机构：

[1] US FDA, Pacific Reg Lab SW, Irvine, CA 92612 USA

[2] US FDA, Denver Dist Lab, Denver, CO 80225 USA

来源：

JOURNAL OF FOOD PROTECTION | 2008年 / 71卷 / 12期

关键词：

D O I：

10.4315/0362-028X-71.12.2436

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conventional methods for detection of Salmonella serovars; in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the Salmonella-specific invA gene. The method has been tested with a total of 384 field-isolated Salmonella serovars and non-Salmonella stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting Salmonella in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU`/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's Bacteriological Analytical Manual cell-culturing and the AOAC International-approved VIDAS methods to detect Salmonella in foods.

引用

页码：2436 / 2441

页数：6

共 33 条

[1] SALMONELLA IDENTIFICATION BY THE POLYMERASE CHAIN-REACTION [J].