Automated sample preparation with extraction columns by means of anti-isoproturon immunosorbents for the determination of phenylurea herbicides in water followed by liquid chromatography diode array detection and liquid chromatography atmospheric pressure chemical ionization mass spectrometry

被引:57
作者
Ferrer, I
Pichon, V
Hennion, MC
Barcelo, D
机构
[1] CSIC,CID,DEPT ENVIRONM CHEM,BARCELONA 08034,SPAIN
[2] ECOLE SUPER PHYS & CHIM IND VILLE PARIS,CHIM ANALYT LAB,URA CNRS 437,F-75231 PARIS 05,FRANCE
关键词
immunoaffinity columns; water analysis; sample handling; pesticides; phenylurea pesticides;
D O I
10.1016/S0021-9673(97)00437-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The retention of five phenylurea herbicides (chlorotoluron, isoproturon, diuron, linuron and diflubenzuron) was evaluated by solid-phase extraction with an automated sample preparation (ASPEC) system using anti-isoproturon immunosorbents. The extraction was carried out after the percolation of 50 mi of LC-grade water and groundwater samples spiked with a mixture of the five pesticides at the ppb level and then elution with 4 mi of a mixture of methanol-water (70:30, v/v) and 1 mi of LC-grade water. The recoveries obtained ranged from 16 to 97% indicating a good affinity of the polyclonal antibodies of the immunosorbent for compounds with similar structures to the antigen pesticide isoproturon. An inter-laboratory study using Aquacheck certified samples was performed in order to validate the use of the immunosorbent for the analysis of environmental water samples. For the groundwater samples the calibration curves were Linear in the range between I and 3 mu g/l for each compound using liquid chromatography with diode array detection (LC-DAD). The overall mean difference comparing the values obtained by this method and the real values given by Aquacheck varied between 1 and 22%. AII samples were analyzed simultaneously by LC-DAD and liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. (C) 1997 Elsevier Science B.V.
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页码:91 / 98
页数:8
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