Optimization of pH and temperature for β-xylosidase recovery by reversed micelles

被引：9

作者：

Hasmann, FA

Pessoa, A

Roberto, IC

机构：

[1] FAENQUIL, Dept Biotechnol, BR-12600000 Lorena, SP, Brazil

[2] Univ Sao Paulo, FCF, Biochem & Pharmaceut Dept, BR-05315970 Sao Paulo, Brazil

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

beta-xylosidase; enzyme recovery; liquid-liquid extraction; reversed micelles; surface response methodology;

D O I：

10.1023/A:1008942428767

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A mathematical model was found to represent the enzyme yield (Y) as a function of pH (X-1) and temperature (X-2): Y = 36.89 + 10.83X(1) - 12.17X(2) - 6X(1)X(2) - 17.24X(1)(2) - 13.24X(2)(2). The optimum values for pH and temperature to attain the maximum b-xylosidase recovery (42%) were 3.3 and 24 degrees C, respectively.

引用

页码：239 / 242

页数：4

共 10 条

[1] MICROBIAL XYLANOLYTIC SYSTEMS [J].