Stabilization of firefly luciferase against thermal stress by osmolytes

被引:36
作者
Mehrabi, Maryam [1 ]
Hosseinkhani, Saman [1 ]
Ghobadi, Sirous [2 ]
机构
[1] Tarbiat Modares Univ, Fac Basic Sci, Dept Biochem, Tehran, Iran
[2] Razi Univ, Fac Sci, Dept Biol, Kermanshah, Iran
关键词
firefly luciferase; osmolytes; stabilization;
D O I
10.1016/j.ijbiomac.2008.05.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of osmolytes, including sucrose, sorbitol and proline on the remaining activity of firefly luciferase were measured. Heat inactivation studies showed that these osmolytes maintain the remaining activity of enzyme and increase activation energy of thermal unfolding reaction. Fluorescence and circular dichroism (CD) experiments showed changes in secondary and tertiary structure of firefly luciferase, in the presence of sucrose, sorbitol and proline. The unfolding curves of luciferase (obtained by far-UV CD spectra), indicated an irreversible thermal denaturation and raising of the midpoint of the unfolding transition temperature (T-m) in the presence of osmolytes. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:187 / 191
页数:5
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