Peptide substrate for caspase-3 with 2-aminoacridone as reporting group

被引：9

作者：

Lozanov, Valentin ^{[1
]}

Ivanov, Ivaylo P. ^{[2
]}

Benkova, Bistra ^{[1
]}

Mitev, Vanio ^{[1
]}

机构：

[1] Med Univ Sofia, Dept Chem & Biochem, Sofia 1431, Bulgaria

[2] Univ Sofia St Kl Ohridsky, Fac Biol, Sofia 1164, Bulgaria

来源：

AMINO ACIDS | 2009年 / 36卷 / 03期

关键词：

2-Aminoacridone; Fluorogenic substrate; Fluorescent substrate; Caspase-3; Apoptosis; PRECOLUMN FLUORESCENCE DERIVATIZATION; APOPTOSIS; ASSAY; PROTEINASES; SPECIFICITY; ACTIVATION; MEMBERS; DESIGN; FAMILY; ENZYME;

D O I：

10.1007/s00726-008-0136-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.

引用

页码：581 / 586

页数：6

共 23 条

[1]

FLUOROMETRIC CONTINUOUS KINETIC ASSAY OF ALPHA-CHYMOTRYPSIN USING NEW PROTEASE SUBSTRATES POSSESSING LONG-WAVE EXCITATION AND EMISSION MAXIMA [J].