Two-photon excited imaging of photosensitizers in tissues

被引：3

作者：

Coleno, ML ^{[1
]}

Wallace, VP ^{[1
]}

Sun, CH ^{[1
]}

Dunn, AK ^{[1
]}

Berns, MW ^{[1
]}

Tromberg, BJ ^{[1
]}

机构：

[1] Univ Calif Irvine, Laser Microbeam Program, Beckman Laser Inst & Med Clin, Irvine, CA 92612 USA

来源：

OPTICAL DIAGNOSTICS AND LIVING CELLS II, PROCEEDINGS OF | 1999年 / 3604卷

关键词：

two-photon microscopy; fluorescence; matrix remodeling; photodynamic therapy; photosensitizers; collagen; fibroblasts; macrophages; wound healing; tissue imaging;

D O I：

10.1117/12.349217

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two-photon microscopy (TPM) is a non-invasive biological imaging technique that can be used to selectively image cellular activity and photosensitizer (PS) localization within highly scattering epithelial tissues at depths of similar to 200 mu m with submicron resolution. The principal objective of this study was to develop a model system for understanding the impact of photodynamic therapy on cellular and extracellular matrix remodeling in biological tissues. An artificial tissue model (RAFT) composed of collagen, embedded fibroblasts, and macrophage cells has been developed for this purpose. TPM is utilized to monitor extracellular matrix remodeling following PDT by imaging collagen/elastin autofluorescence. Selective uptake of photosensitizers by specific cellular components in the matrix can also be visualized by TPM.

引用

页码：67 / 73

页数：7