Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants by Use of Dried Blood Spots and an Ultrasensitive p24 Antigen Assay

被引：16

作者：

Cachafeiro, Ada ^{[1
]}

Sherman, Gayle G. ^{[2
]}

Sohn, Annette H. ^{[3
]}

Beck-Sague, Consuelo ^{[4
]}

Fiscus, Susan A. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA

[2] Univ Witwatersrand, Dept Mol Med & Haematol, Johannesburg, South Africa

[3] Univ Calif San Francisco, Div Pediat Infect Dis, San Francisco, CA 94143 USA

[4] Clin Familia MIR, La Romana, Dominican Rep

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 02期

关键词：

POLYMERASE-CHAIN-REACTION; LOW-RESOURCE SETTINGS; FILTER-PAPER; HIV-INFECTION; WHOLE-BLOOD; PCR ASSAYS; TRANSMISSION; DNA; RNA; SPECIMENS;

D O I：

10.1128/JCM.01181-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We tested 617 dried blood spots (DBS) from human immunodeficiency virus-exposed infants from five countries using an ultrasensitive p24 antigen assay (Up24). The sensitivity was 94.4% (67/71) and the specificity was 100% (431/431) for infants with DBS specimens <= 20 months old; DBS older than 30 months demonstrated only 72.2% sensitivity (39/54) (P < 0.001) but displayed 100% specificity (61/61).

引用

页码：459 / 462

页数：4

共 32 条

[1] Rational testing of the HIV-exposed infant [J].

Benjamin, DK ;

Miller, WC ;

Fiscus, SA ;

Benjamin, DK ;

Morse, M ;

Valentine, M ;

McKinney, RE .

PEDIATRICS, 2001, 108 (01)

[2] Blood collection on filter paper: A practical approach to sample collection for studies of perinatal HIV transmission [J].

Biggar, RJ ;

Miley, W ;

Miotti, P ;

Taha, TE ;

Butcher, A ;

Spadoro, J ;

Waters, D .

JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY, 1997, 14 (04) :368-373

[3]

BredbergRaden U, 1996, J ACQ IMMUN DEF SYND, V12, P421

[4] RAPID SCREENING FOR EARLY DETECTION OF MOTHER-TO-CHILD TRANSMISSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

CASSOL, S ;

BUTCHER, A ;

KINARD, S ;

SPADORO, J ;

SY, T ;

LAPOINTE, N ;

READ, S ;

GOMEZ, P ;

FAUVEL, M ;

MAJOR, C ;

OSHAUGHNESSY, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (11) :2641-2645

[5] USE OF DRIED BLOOD SPOT SPECIMENS IN THE DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY THE POLYMERASE CHAIN-REACTION [J].

CASSOL, S ;

SALAS, T ;

ARELLA, M ;

NEUMANN, P ;

SCHECHTER, MT ;

OSHAUGHNESSY, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (04) :667-671

[6]

CASSOL SA, 1992, J ACQ IMMUN DEF SYND, V5, P113

[7] IDENTIFYING HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION AT BIRTH - APPLICATION OF POLYMERASE CHAIN-REACTION TO GUTHRIE CARDS [J].

COMEAU, AM ;

HSU, HW ;

SCHWERZLER, M ;

MUSHINSKY, G ;

WALTER, E ;

HOFMAN, L ;

GRADY, GF .

JOURNAL OF PEDIATRICS, 1993, 123 (02) :252-258

[8]

COMEAU AM, 1992, J ACQ IMMUN DEF SYND, V5, P271

[9] Comparison of human immunodeficiency virus 1 DNA polymerase chain reaction and qualitative and quantitative RNA polymerase chain reaction in human immunodeficiency virus 1-exposed infants [J].

Cunningham, CK ;

Charbonneau, TT ;

Song, KM ;

Patterson, D ;

Sullivan, T ;

Cummins, T ;

Poiesz, B .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1999, 18 (01) :30-35

[10] Pediatric human immunodeficiency virus screening in an African district hospital [J].

De Baets, AJ ;

Edidi, BS ;

Kasali, MJ ;

Beelaert, G ;

Schrooten, W ;

Litzroth, A ;

Kolsteren, P ;

Denolf, D ;

Fransen, K .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (01) :86-92

← 1 2 3 4 →