Alteration of integrin expression relates to malignant progression of human papillomavirus-immortalized esophageal keratinocytes

被引：17

作者：

Sashiyama, H

Shino, Y

Sakao, S

Shimada, H

Kobayashi, S

Ochiai, T

Shirasawa, H

机构：

[1] Chiba Univ, Dept Mol Virol, Grad Sch Med, Chiba, Japan

[2] Chiba Univ, Grad Sch Med, Acad Dept Surg, Chiba, Japan

来源：

CANCER LETTERS | 2002年 / 177卷 / 01期

关键词：

HPV type 16; E6; E7; human esophageal epithelial cells; integrin; integrin alpha 6 beta 4;

D O I：

10.1016/S0304-3835(01)00771-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To investigate cellular changes related to the malignant progression of keratinocytes, we studied the serum-resistant clones from CHEK-1, a human papillomavirus type 16 E6/E7-immortalized esophageal cell line cultured in a serum-free medium. Established clones exhibited morphologic variety. Slow growing clones presented in cuboidal shapes with tight cellular adhesion and highly expressed alpha2 and alpha6beta4 integrins. Moderately proliferating clones showed loose intercellular adhesion and reduced expression of a2 integrin. Spindle-shaped, rapidly proliferating clones with prominent actin stress fibers demonstrated reduced alpha6 and alpha4 integrin expression in addition to alpha2 integrin and showed anchorage-independent growth. Reduced expression of alpha2 integrin was observed between 50 and 100 population doubling lengths (PDLs) during the immortalization of CHEK-1. These results suggest that the reductions of alpha2 and alpha6beta4 integrins are related to changes seen during immortalization and malignant progression. (C) 2002 Elsevier Science Ireland Ltd. All fights reserved.

引用

页码：21 / 28

页数：8

共 27 条

[1] EXPRESSION OF BETA-1-INTEGRIN, BETA-3-INTEGRIN, BETA-4-INTEGRIN AND BETA-5-INTEGRIN BY HUMAN EPIDERMAL-KERATINOCYTES AND NONDIFFERENTIATING KERATINOCYTES [J].