Sequential Limb Ischemia Demonstrates Remote Postconditioning Protection of Murine Skeletal Muscle

被引:19
作者
Eberlin, Kyle R. [1 ]
McCormack, Michael C. [1 ]
Nguyen, John T. [1 ]
Tatlidede, H. Soner [1 ]
Randolph, Mark A. [1 ]
Austen, William G., Jr. [1 ]
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Plast Surg Res Lab,Div Plast Surg, Boston, MA 02114 USA
关键词
REPERFUSION INJURY; ADENOSINE RECEPTORS; RENAL ISCHEMIA; FLAP SURVIVAL; INFARCT SIZE; MYOCARDIUM; ACTIVATION; MODEL; COMPLEMENT; MECHANISMS;
D O I
10.1097/PRS.0b013e318191bcd6
中图分类号
R61 [外科手术学];
学科分类号
100210 [外科学];
摘要
Background: Ischemic postconditioning, the process of exposing tissues to brief cycles of ischemia-reperfusion after critical ischemia, can mitigate local ischemia-reperfusion injury. Remote protection of skeletal muscle has never been demonstrated in postconditioning models of ischemia-reperfusion injury. Methods: Mice were subjected to 2 hours of ipsilateral hind limb ischemia followed by reperfusion. Contralateral limb ischemia was subsequently induced for 2 hours after either 0 (n = 6), 20 (n = 6), or 120 (n = 5) minutes of ipsilateral limb reperfusion. These groups were compared with animals subjected to bilateral simultaneous injury (n = 8) and sham animals that did not undergo ischemia (n = 6). The gastrocnemius muscles were harvested for histologic evaluation, and injury was recorded as the percentage of injured fibers. Results: The first limbs undergoing injury in the 20-minute interval group had a 59 percent injury reduction compared with contralateral limbs (16.0 +/- 2.4 percent versus 39.5 +/- 6.5 percent) after 24 hours of reperfusion and 62 percent reduction after 48 hours (24.4 +/- 3.0 percent versus 63.6 +/- 5.5 percent). In animals with no interval or a 120-minute interval between the onset of limb ischemia, there was no significant difference in injury between hind limbs. The injury in these groups was similar to that in hind limbs subjected to simultaneous bilateral ischemia. Conclusions: A 20-minute reperfusion interval between hind limb ischemia significantly protects against injury in the initially ischemic limb, while similar injury is observed with simultaneous ischemia or an interval of 120 minutes. This study demonstrates remote postconditioning of skeletal muscle and may lead to the development of post hoc therapies. (Plast. Reconstr. Surg. 123 (Suppl.): 8S, 2009.)
引用
收藏
页码:8S / 16S
页数:9
相关论文
共 44 条
[1]
Mast cell protease 5 mediates ischemia-reperfusion injury of mouse skeletal muscle [J].
Abonia, JP ;
Friend, DS ;
Austen, WG ;
Moore, FD ;
Carroll, MC ;
Chan, R ;
Afnan, J ;
Humbles, A ;
Gerard, C ;
Knight, P ;
Kanaoka, Y ;
Yasuda, S ;
Morokawa, N ;
Austen, KF ;
Stevens, RL ;
Gurish, MF .
JOURNAL OF IMMUNOLOGY, 2005, 174 (11) :7285-7291
[2]
Murine hindlimb reperfusion injury can be initiated by a self-reactive monoclonal IgM [J].
Austen, WG ;
Zhang, M ;
Chan, R ;
Friend, D ;
Hechtman, HB ;
Carroll, MC ;
Moore, FD .
SURGERY, 2004, 136 (02) :401-406
[3]
Intestinal ischemia-reperfusion injury is mediated by the membrane attack complex [J].
Austen, WG ;
Kyriakides, C ;
Favuzza, J ;
Wang, Y ;
Kohzik, L ;
Moore, FD ;
Hechtman, HB .
SURGERY, 1999, 126 (02) :343-348
[4]
Baumeister SP, 2004, J RECONSTR MICROSURG, V20, P253
[5]
A noninvasive murine model of hind limb ischemia-reperfusion injury [J].
Bonheur, JA ;
Albadawi, H ;
Patton, GM ;
Watkins, MT .
JOURNAL OF SURGICAL RESEARCH, 2004, 116 (01) :55-63
[6]
ROLE OF CYTOCHROME-P-450 IN REPERFUSION INJURY OF THE RABBIT LUNG [J].
BYSANI, GK ;
KENNEDY, TP ;
KY, N ;
RAO, NV ;
BLAZE, CA ;
HOIDAL, JR .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (05) :1434-1441
[7]
Acute ischemic preconditioning of skeletal muscle prior to flap elevation augments muscle-flap survival [J].
Carroll, CMA ;
Carroll, SM ;
Overgoor, MLE ;
Tobin, G ;
Barker, JH .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1997, 100 (01) :58-65
[8]
Reperfusion injury to skeletal muscle affects primarily type II muscle fibers [J].
Chan, RK ;
Austen, WG ;
Ibrahim, S ;
Ding, GY ;
Verna, N ;
Hechtman, HB ;
Moore, FD .
JOURNAL OF SURGICAL RESEARCH, 2004, 122 (01) :54-60
[9]
Ischaemia-reperfusion is an event triggered by immune complexes and complement [J].
Chan, RK ;
Ibrahim, SI ;
Verna, N ;
Carroll, M ;
Moore, FD ;
Hechtman, HB .
BRITISH JOURNAL OF SURGERY, 2003, 90 (12) :1470-1478
[10]
Randomized controlled trial of the effects of remote ischemic preconditioning on children undergoing cardiac surgery - First clinical application in humans [J].
Cheung, Michael M. H. ;
Kharbanda, Rajesh K. ;
Konstantinov, Igor E. ;
Shimizu, Mikiko ;
Frndova, Helena ;
Li, Jia ;
Holtby, Helen M. ;
Cox, Peter N. ;
Smallhorn, Jeffrey F. ;
Van Arsdell, Glen S. ;
Redington, Andrew N. .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2006, 47 (11) :2277-2282