Glassy carbon paste electrodes modified with polyphenol oxidase -: Analytical applications

被引：53

作者：

Rodríguez, MC ^{[1
]}

Rivas, GA ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Fis, IFIQC, RA-5000 Cordoba, Argentina

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 459卷 / 01期

关键词：

glassy carbon microparticles; glassy carbon paste electrode; composite electrodes; enzymatic biosensors; phenol biosensors; catechol biosensors;

D O I：

10.1016/S0003-2670(02)00088-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical behavior of a glassy carbon paste electrode (GCPE) is evaluated in comparison to that of graphite paste electrode (gPE) and glassy carbon electrode (GCE). Important shifting in the peak potentials and increases in the peak currents for catechol, ascorbic acid, dopamine and hydroquinone were obtained for the GCPE and its usefulness for the development of phenol and catechol biosensors was also evaluated. Both, pure mushroom polyphenol oxidase (PPO) and fresh mushroom tissues were used as biorecognition elements. The effect of the binder percentage in the composite material was also studied. The bioelectrode was used for the determination of dopamine and acetaminophen in pharmaceutical formulations and for the detection of polyphenols in wine and tea. The bioelectrode demonstrated to be very stable as the response remained around 90% after four months at 4 degreesC. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：43 / 51

页数：9

共 15 条

[1] ELECTROCHEMICAL ACTIVATION OF CARBON ELECTRODES IN BASE - MINIMIZATION OF DOPAMINE ADSORPTION AND ELECTRODE CAPACITANCE [J].

ANJO, DM ;

KAHR, M ;

KHODABAKHSH, MM ;

NOWINSKI, S ;

WANGER, M .

ANALYTICAL CHEMISTRY, 1989, 61 (23) :2603-2608

[2]

BARBERO C, 1993, J ELECTROCHEM SOC, V140, P1, DOI 10.1149/1.2056086

[3] A PYROLYTIC CARBON-FILM ELECTRODE FOR VOLTAMMETRY .5. CHARACTERIZATION AND COMPARISON WITH THE GLASSY-CARBON ELECTRODE BY ELECTROCHEMICAL PRETREATMENT IN BASIC SOLUTION [J].

BEILBY, AL ;

CARLSSON, A .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1988, 248 (02) :283-304

[4] ELECTROCHEMICAL PRETREATMENT OF CARBON ELECTRODES AS A FUNCTION OF POTENTIAL, PH, AND TIME [J].

BEILBY, AL ;

SASAKI, TA ;

STERN, HM .

ANALYTICAL CHEMISTRY, 1995, 67 (05) :976-980

[5]

BRUN A, 1974, J ELECTROANAL CHEM, V49, P287, DOI 10.1016/S0022-0728(74)80236-6

[6] BIOCATALYSIS WITH POLYPHENOL OXIDASE - A REVIEW [J].

BURTON, SG .

CATALYSIS TODAY, 1994, 22 (03) :459-487

[7] ELECTRON-TRANSFER KINETICS AT MODIFIED CARBON ELECTRODE SURFACES - THE ROLE OF SPECIFIC SURFACE SITES [J].

CHEN, PH ;

FRYLING, MA ;

MCCREERY, RL .

ANALYTICAL CHEMISTRY, 1995, 67 (18) :3115-3122

[8]

DEDDON BJ, 1997, ELECTROCHIM ACTA, V42, P1883

[9] Amperometric determination of dopamine on vegetal-tissue enzymatic electrodes. Analysis of interferents and enzymatic selectivity [J].

Forzani, ES ;

Rivas, GA ;

Solis, VM .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 435 (1-2) :77-84

[10] CARBON-PASTE ELECTRODES MODIFIED WITH ENZYMES, TISSUES, AND CELLS [J].

GORTON, L .

ELECTROANALYSIS, 1995, 7 (01) :23-45

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