Translationally controlled tumor protein: A protein identified in several nontumoral cells including erythrocytes

被引：128

作者：

Sanchez, JC

Schaller, D

Ravier, F

Golaz, O

Jaccoud, S

Belet, M

Wilkins, MR

James, R

Deshusses, J

Hochstrasser, D

机构：

[1] UNIV HOSP GENEVA,CLIN CHEM LAB,GENEVA,SWITZERLAND

[2] UNIV GENEVA,DEPT BIOCHEM,CH-1211 GENEVA,SWITZERLAND

[3] UNIV HOSP GENEVA,DIABETOL DEPT,GENEVA,SWITZERLAND

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 01期

关键词：

calcium-binding protein; two-dimensional polyacrylamide gel electrophoresis; growth related protein; post-translational modification; POLYVINYLIDENE DIFLUORIDE MEMBRANES; EHRLICH ASCITES TUMOR; MESSENGER-RNA; 2-DIMENSIONAL ELECTROPHORESIS; POLYACRYLAMIDE GELS; SEQUENCE; SEPARATION;

D O I：

10.1002/elps.1150180127

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The translationally controlled tumor protein (TCTP) is a growth-related protein which is regulated at the translational level. It is present in mammals, higher plants and Saccharomyces cerevisiae. This study was undertaken to localize and further characterize the TCTP in human cell lysates using two-dimensional gel electrophoresis, monoclonal antibodies, and Ca-45-gel overlay. TCTP was found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It could not be detected in kidney and renal cell carcinoma (RCC). A monoclonal antibody raised against TCTP detected three isoforms likely due to post-translational modifications. A calcium binding property was found as well as heat stability and cytoplasmic localization. The high degree of homology from plants to man and its expression in many tissues suggests that TCTP most likely has a cell housekeeping function.

引用

页码：150 / 155

页数：6

共 22 条

[1]

BAIROCH A, 1994, NUCLEIC ACIDS RES, V22, P3578

[2]

BIELKA H, 1988, BIOMED BIOCHIM ACTA, V47, P557

[3] MICROPREPARATIVE 2-DIMENSIONAL ELECTROPHORESIS ALLOWING THE SEPARATION OF SAMPLES CONTAINING MILLIGRAM AMOUNTS OF PROTEINS [J].