Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

被引：18

作者：

Bach, HJ ^{[1
]}

Hartmann, A

Trevors, JT

Munch, JC

机构：

[1] GSF, Natl Res Ctr Environm & Hlth, Inst Soil Ecol, D-85764 Neuherberg, Germany

[2] Univ Guelph, Dept Environm Biol, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1999年 / 37卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Bacillus cereus; magnetic capture-hybridization; neutral protease; prokaryotic mRNA;

D O I：

10.1016/S0167-7012(99)00066-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：187 / 192

页数：6

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