Involvement of the transcriptional factor E2F1 in the regulation of the rRNA promoter

被引：18

作者：

Ayrault, O ^{[1
]}

Andrique, L ^{[1
]}

Séité, P ^{[1
]}

机构：

[1] Oncol Mol Lab, F-86022 Poitiers, France

来源：

EXPERIMENTAL CELL RESEARCH | 2006年 / 312卷 / 07期

关键词：

E2F1; p14(ARF); rRNA promoter; ChIP; gel shift;

D O I：

10.1016/j.yexcr.2006.01.027

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

p16(INK4a) -pRB-E2F and ARF-MDM2-p53 are two major tumor suppressor networks involved in cell proliferation control. The nucleolar ARF protein binds to MDM2 to activate the growth suppressive functions of p53, but can also exert its tumor suppressor activity independently of p53, through mechanisms involving other regulators: in that manner, p14(ARF) has been shown to inhibit the transcriptional activity of E2F1 in vitro, suggesting that the two pathways intersect with one another. More recently, ARF has been shown to inhibit ribosomal RNA processing, and to specifically interact with the rRNA promoter, suggesting a role in the regulation of both maturation and transcription processes. We show here that E2F1 can bind the rRNA promoter and modulate its activity through the interaction with two E2F1-binding sequences we have identified. The regulation of ribosome biogenesis appears as a major p53-independent process, which involves both ARF and E2F1 to control cell proliferation. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1185 / 1193

页数：9

共 34 条

[1] Distinct recruitment of E2F family members to specific E2F-binding sites mediates activation and repression of the E2F1 promoter [J].