MRMer, an Interactive Open Source and Cross-platform System for Data Extraction and Visualization of Multiple Reaction Monitoring Experiments

被引：59

作者：

Martin, Daniel B. ^{[2
]}

Holzman, Ted ^{[1
]}

May, Damon ^{[1
]}

Peterson, Amelia ^{[2
]}

Eastham, Ashley ^{[2
]}

Eng, Jimmy ^{[2
,3
]}

McIntosh, Martin ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Seattle, WA 98112 USA

[2] Inst Syst Biol, Seattle, WA 98103 USA

[3] Univ Washington Med S Lake Union, Seattle, WA 98109 USA

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2008年 / 7卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1074/mcp.M700504-MCP200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple reaction monitoring (MRM) mass spectrometry identifies and quantifies specific peptides in a complex mixture with very high sensitivity and speed and thus has promise for the high throughput screening of clinical samples for candidate biomarkers. We have developed an interactive software platform, called MRMer, for managing highly complex MRM-MS experiments, including quantitative analyses using heavy/light isotopic peptide pairs. MRMer parses and extracts information from MS files encoded in the platform-independent mzXML data format. It extracts and infers precursor-product ion transition pairings, computes integrated ion intensities, and permits rapid visual curation for analyses exceeding 1000 precursor-product pairs. Results can be easily output for quantitative comparison of consecutive runs. Additionally MRMer incorporates features that permit the quantitative analysis experiments including heavy and light isotopic peptide pairs. MRMer is open source and provided under the Apache 2.0 license. Molecular & Cellular Proteomics 7: 2270-2278, 2008.

引用

页码：2270 / 2278

页数：9

共 23 条

[1] Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins [J].