Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases

被引：335

作者：

Feig, LA ^{[1
]}

机构：

[1] Tufts Univ, Sch Med, Dept Biochem, Boston, MA 02110 USA

来源：

NATURE CELL BIOLOGY | 1999年 / 1卷 / 02期

关键词：

D O I：

10.1038/10018

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

One of the most powerful ways of studying the function of a protein is to specifically block its activity within cells. Over the past decade, dominant-inhibitory proteins have emerged as popular tools with which to accomplish this task; these mutated proteins interfere with the function of their normal cellular counterparts or with proteins that interact with them. This approach has been used extensively in the elucidation of signal-transduction cascades, such as those involving Has-family proteins. Here I discuss the power and potential pitfalls of using dominant-inhibitory Has proteins.

引用

页码：E25 / E27

页数：3

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