Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii

被引：96

作者：

Wang, HX

Ng, TB ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Fac Med, Shatin, Hong Kong, Peoples R China

[2] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

[3] China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 69卷 / 05期

关键词：

D O I：

10.1007/s00253-005-0086-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A purification scheme involving ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose, followed by fast protein liquid chromatography-gel filtration on Superdex 75, was utilized to isolate a laccase from the fruiting bodies of the mushroom Pleurotus eryngii. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose and unadsorbed on CM-cellulose. The molecular mass of the laccase in sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 34 kDa. The laccase activity was maximal at 70 degrees C and remained high at 80 degrees C. High activity of the laccase was maintained at ph 3 to 5. The activity underwent an abrupt decline at pH 6 and 7 and was indiscernible at ph 8 and 9. The laccase exhibited an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 2.2 mu M. Its N-terminal sequence was dissimilar to those of laccase isoenzymes previously isolated from cultured mycelia of the same species.

引用

页码：521 / 525

页数：5

共 25 条

[1] Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus [J].