RNA-Seq profiling of circular RNAs in human laryngeal squamous cell carcinomas

被引：72

作者：

Lu, Cheng ^{[1
]}

Shi, Xi ^{[2
]}

Wang, Amanda Y. ^{[3
,4
]}

Tao, Yuan ^{[5
]}

Wang, Zhenxiao ^{[1
]}

Huang, Chaoping ^{[1
]}

Qiao, Yuehua ^{[2
]}

Hu, Hongyi ^{[5
]}

Liu, Liangfa ^{[1
]}

机构：

[1] Capital Med Univ, Beijing Friendship Hosp, Dept Otolaryngol Head & Neck Surg, 95th Yongan Rd, Beijing 100050, Peoples R China

[2] Xuzhou Med Coll, Inst Audiol & Speech Sci, Xuzhou 221004, Peoples R China

[3] UNSW, George Inst Global Hlth, Renal & Metab Div, Sydney, NSW, Australia

[4] Macquarie Univ, Fac Med & Hlth Sci, Sydney, NSW 2109, Australia

[5] Peking Univ, Shenzhen Hosp, Dept Otolaryngol, Shenzhen 518036, Peoples R China

来源：

MOLECULAR CANCER | 2018年 / 17卷

关键词：

Circular RNAs (circRNAs); RNA-sequencing (RNA-Seq); Laryngeal squamous cell carcinomas (LSCC); CANCER; EXPRESSION; HEAD;

D O I：

10.1186/s12943-018-0833-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Abnormal expression of non-coding circular RNAs (circRNAs) have been reported in many types of tumors. circRNA have been suggested to be an ideal candidate biomarker for diagnostic and therapeutic implications in cancers. The aim of this study was to assess the circRNA expression profile of laryngeal squamous cell carcinomas (LSCC). The biopsy samples from patients with LSCC were obtained intra-operatively. The circRNA expression was performed using secondary sequencing. Among 10 patients with LSCC, 2 were well differentiated, 3 were moderately differentiated and 5 were adjunctive samples with normal and LSCC tissues. A total of 21,444 distinct circRNA candidates were detected. Among them, we defined the statistical criteria for selecting aberrant-expressed circRNA using a q-value of < 0.001 with a fold change of > 2.0 or < 0.5. A total of 29 circRNA were upregulated and 19 circRNA were downregulated significantly in the LSCC tissues. The intersection of these dysregulated circRNAs of normal-well differentiated set and normal-moderately differentiated set was then assessed to narrow the upregulated and downregulated circRNAs down to 18 and 5 respectively. Furthermore, an association of the circRNA-miRNA-mRNA was investigated, showing that 20 dysregulated circRNA successfully predicted an interaction with several cancer-related miRNAs. Finally, a further KEGG analysis showed that PPAR, Axon guidance, Wnt and Cell cycle signaling pathway were key putative pathways in the process of LSCC. hsa_circ:chr20:31876585-31,897,648 was found to be able to differentiate most of LSCC from the matching normal tissues. This observational study demonstrated dysregulation of circRNA in LSCC, which may have an impact on development of potential biomarkers in this disease. Validation of down-regulation of hsa_circ:chr20:31876585-31,897,648 in LSCC compared to each adjunctive tissue by Q-RT-PCR, indicating that hsa_circ:chr20:31876585-31,897,648 may be a novel promising tumor suppresser in LSCC.

引用

页数：9

共 10 条

[1]

Stem cell marker (Nanog) and Stat-3 signaling promote MicroRNA-21 expression and chemoresistance in hyaluronan/CD44-activated head and neck squamous cell carcinoma cells [J].