Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays

被引:70
作者
Liao, R. S. [1 ,2 ]
Tomalty, L. L. [1 ,2 ]
Majury, A. [1 ]
Zoutman, D. E. [1 ,2 ]
机构
[1] Queens Univ, Dept Microbiol & Immunol, Kingston, ON K7L 3N6, Canada
[2] Queens Univ, Dept Pathol & Mol Med, Div Med Microbiol & Infect Control, Kingston, ON, Canada
关键词
DIRECTIGEN FLU-A+B; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; DIRECT FLUORESCENT ASSAY; SHELL VIAL CULTURE; RAPID DETECTION; B VIRUSES; ENZYME-IMMUNOASSAY; CELL-CULTURE; BINAX-NOW;
D O I
10.1128/JCM.01213-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the Smart-Cycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A + B and Binax NOW RSV assays over two successive respiratory virus seasons. Ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 119 were positive for RSV and 59 were positive for influenza virus. The median age of the patients whose specimens were positive for RSV and influenza virus were 6.3 months and 42.4 years, respectively. The specificity of all of the methods tested was >= 99%, and the individual sensitivities of NOW RSV, RSV culture, Directigen A + B, influenza virus culture, and the Proflu-1 PCR for influenza/RSV were 82% (95% confidence interval [CI], 73 to 88), 57% (95% CI, 44 to 69), 59% (95% CI, 44 to 72), 54% (95% CI, 38 to 69), and 98% (95% CI, 93 to 100)/95% (95% CI, 85 to 99), respectively. In a clinical setting where viral isolation is performed to confirm rapid antigen immunoassay results for these common respiratory viruses, one-step real-time reverse transcriptase PCR testing can be a more sensitive and timely confirmatory method.
引用
收藏
页码:527 / 532
页数:6
相关论文
共 48 条
[1]   Evaluation of the Quidel QuickVue test for detection of influenza A and B viruses in the pediatric emergency medicine setting by use of three specimen collection methods [J].
Agoritsas, Konstantinos ;
Mack, Kathy ;
Bonsu, Bema K. ;
Goodman, Debbie ;
Salamon, Douglas ;
Marcon, Mario J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (07) :2638-2641
[2]   A comparison of BinaX™ NOW® to viral culture and direct fluorescent assay testing for respiratory syncytial virus [J].
Aldous, WK ;
Gerber, K ;
Taggart, EW ;
Thomas, J ;
Tidwell, D ;
Daly, JA .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2004, 49 (04) :265-268
[3]   Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study [J].
Barenfanger, J ;
Drake, C ;
Leon, N ;
Mueller, T ;
Troutt, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (08) :2824-2828
[4]   Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses [J].
Boivin, G ;
Côté, S ;
Déry, P ;
De Serres, G ;
Bergeron, MG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) :45-51
[5]   Impact of the rapid diagnosis of influenza on physician decision-making and patient management in the pediatric emergency department: Results of a randomized, prospective, controlled trial [J].
Bonner, AB ;
Monroe, KW ;
Talley, LI ;
Klasner, AE ;
Kimberlin, DW .
PEDIATRICS, 2003, 112 (02) :363-367
[6]   Respiratory syncytial virus detection by remel Xpect, Binax Now RSV, direct immunofluorescent staining, and tissue culture [J].
Borek, AP ;
Clemens, SH ;
Gaskins, VK ;
Aird, DZ ;
Valsamakis, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (03) :1105-1107
[7]   Comparison of the directigen flu A+B membrane enzyme immunoassay with viral culture for rapid detection of influenza A and B viruses in respiratory specimens [J].
Cazacu, AC ;
Chung, SE ;
Greer, J ;
Demmler, GJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3707-3710
[8]   Evaluation of the Directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections [J].
Chan, KH ;
Maldeis, N ;
Pope, W ;
Yup, A ;
Ozinskas, A ;
Gill, J ;
Seto, WH ;
Shortridge, KF ;
Peiris, JSM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1675-1680
[9]  
Collins PL., 2007, FIELDS VIROLOGY, V2, P1601
[10]   Performance of a rapid assay (Binax NOW) for detection of respiratory syncytial virus at a children's hospital over a 3-year period [J].
Cruz, Andrea T. ;
Cazacu, Andreea C. ;
Greer, Jewel M. ;
Demmler, Gail J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :1993-1995