Chitinous component of the cell wall of Fusarium oxysporum, its structure deduced from chitosanase digestion

被引：16

作者：

Fukamizo, T ^{[1
]}

Honda, Y ^{[1
]}

Toyoda, H ^{[1
]}

Ouchi, S ^{[1
]}

Goto, S ^{[1
]}

机构：

[1] KINKI UNIV, FAC AGR, PLANT PATHOL LAB, NARA 631, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 10期

关键词：

Fusarium oxysporum; cell wall structure; chitosanase; chitooligosaccharide; C-13-NMR;

D O I：

10.1271/bbb.60.1705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cell wall of Fusarium oxysporum was digested with commercial Bacillus pumilus chitosanase, The chitosanase produced low molecular weight heterooligosaccharides consisting of GlcN and GlcNAc from the cell wall, A main component of the digestion products was identified as 2-amino-2-deoxy-beta-D-glucopyranosyl-(1 --> 4)-2-acetamido-2-deoxy-D-glucopyranose The chitosanase appeared to be more effective than Streptomyces griseus chitinase for cell wall digestion, Moreover, maltose was unexpectedly found in the digestion products, indicating that the cell wall contains alpha-1,4-linked glucan chain as a polysaccharide component.

引用

页码：1705 / 1708

页数：4

共 7 条

[1] REACTION-MECHANISM OF CHITOSANASE FROM STREPTOMYCES SP N174 [J].

FUKAMIZO, T ;

HONDA, Y ;

GOTO, S ;

BOUCHER, I ;

BRZEZINSKI, R .

BIOCHEMICAL JOURNAL, 1995, 311 :377-383

[2] SPECIFICITY OF CHITOSANASE FROM BACILLUS-PUMILUS [J].

FUKAMIZO, T ;

OHKAWA, T ;

IKEDA, Y ;

GOTO, S .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1994, 1205 (02) :183-188

[3] SOLID-STATE C-13-NMR ANALYSIS OF CELL-WALL COMPONENTS OF FUSARIUM-OXYSPORUM [J].

FUKAMIZO, T ;

SONODA, K ;

TOYODA, H ;

OUCHI, S ;

GOTO, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (10) :2761-2762

[4] CHITINOUS COMPONENTS OF THE CELL-WALL OF FUSARIUM-OXYSPORUM [J].

FUKAMIZO, T ;

OHKAWA, T ;

SONODA, K ;

TOYODA, H ;

NISHIGUCHI, T ;

OUCHI, S ;

GOTO, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (10) :1632-1636

[5]

OUCHI S, 1992, NATO ADV SCI I A-LIF, V230, P335

[6] PLANT CHITINASES ARE POTENT INHIBITORS OF FUNGAL GROWTH [J].

SCHLUMBAUM, A ;

MAUCH, F ;

VOGELI, U ;

BOLLER, T .

NATURE, 1986, 324 (6095) :365-367

[7] MODE OF INHIBITION OF RHIZOCTONIA-SOLANI IN CHITIN-AMENDED SOIL [J].

SNEH, B ;

KATAN, J ;

HENIS, Y .

PHYTOPATHOLOGY, 1971, 61 (09) :1113-&

← 1 →