In situ localization of yeast artificial chromosome sequences on tomato and potato metaphase chromosomes

被引:12
作者
Fuchs, J
Kloos, DU
Ganal, MW
Schubert, I
机构
[1] Inst. F. Pflanzengenetik K.
关键词
chromosome specific repetitive sequence; fluorescence in situ hybridization; potato; tomato; yeast artificial chromosome;
D O I
10.1007/BF02263677
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In situ localization of short low- or single-copy sequences is still difficult in plants. One solution to this problem could be the use of large yeast artificial chromosomes (YACs) for fluorescence in site hybridization. Two YACs specific for a single copy marker on the long arm of the NOR-chromosome 2 of tomato (Lycopersicon escolentum) were selected. Both probes hybridized exclusively to this chromosome, although one produced a slightly dispersed hybridization signal. Hybridization of these YACs onto potato chromosomes showed a clear single locus on the homoeologous potato chromosome in both cases.
引用
收藏
页码:277 / 281
页数:5
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