Surface planar bilayers of phospholipids used in protein membrane reconstitution:: An atomic force microscopy study

被引：26

作者：

Doménech, O

Merino-Montero, S

Montero, MT

Hernández-Borrell, J

机构：

[1] Univ Barcelona, Dept Fisicoquim, E-08028 Barcelona, Spain

[2] Univ Barcelona, Dept Quim Fis, E-08028 Barcelona, Spain

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2006年 / 47卷 / 01期

关键词：

surface planar bilayers; AFM; phospholipids;

D O I：

10.1016/j.colsurfb.2005.11.025

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, using atomic force microscopy (AFM), we have studied the influence of the temperature on the properties of the surface planar bilayers (SPBs) formed with: (i) the total lipid extract of Escherichia coli; (ii) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPC) (1: 1, mol/mol); and, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol-amine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3: 1, mol/mol). According to the height profile analysis we performed, the height of the SPBs of DMPC:POPC were temperature dependent. Separated domains were observed in the SPBs of the POPE:POPG mixture and the E. coli lipid extract. The implication of those domains for the correct insertion of membrane proteins into proteoliposomes is discussed. (c) 2005 Elsevier B.V. All rights reserved.

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页码：102 / 106

页数：5

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