Rapid identification and quantitative analysis of the chemical constituents in Scutellaria indica L. by UHPLC-QTOE-MS and UHPLC-MS/MS

被引:42
作者
He, Lili [1 ]
Zhang, Zhifeng [2 ]
Lu, Luyang [2 ]
Liu, Yuan [2 ]
Li, Shan [2 ]
Wang, Jiangang [3 ]
Song, Zhijun [1 ]
Yan, Zhigang [1 ]
Miao, Jianhua [1 ]
机构
[1] Guangxi Bot Garden Med Plants, Natl Engn Inst Res & Dev Endangered Med Resources, Nanning, Guangxi Provinc, Peoples R China
[2] Southwest Univ Nationalities, Inst Qinghai Tibetan Plateau, Chengdu 610041, Sichuan Provinc, Peoples R China
[3] Guangzhou Univ Chinese Med, Sch Chinese Mat Med, Guangzhou, Guangdong, Peoples R China
关键词
Scutellaria indica; Scutellarin; Chemical compositions; UHPLC-QTOF-MS; UHPLC-MS/MS; OF-FLIGHT MASS; FLAVONOID CONSTITUENTS; ELECTROSPRAY-IONIZATION; ROOT; QUANTIFICATION; SPECTROMETRY;
D O I
10.1016/j.jpba.2015.08.034
中图分类号
O65 [分析化学];
学科分类号
070302 [分析化学];
摘要
Ultra high performance liquid chromatography (UHPLC) coupled with a hybrid quadrupole time-of-flight mass spectrometry (QTOF-MS) was used to separate and identify the compounds in Scutellaria indica L Fragmentation patterns of these compounds were investigated by the UHPLC-QTOF-MS technique in both negative and positive ion modes. By using this strategy, both intact precursors and fragment ions information were obtained from a single injection. A total of 42 phenolic and other compounds were unequivocally or tentatively identified from S. indica for the first time. Among them, 8 phenolic compounds: scutellarin, luteolin, naringenin, wogonoside, apigenin, hispidulin, wogonin and chrysin were further quantified and used as marker substances by multiple-reaction monitoring in negative ionization mode. The results demonstrated that the calibration curves for all analytes showed good linearity (R-2>0.9992) within the test ranges. The overall limits of detection and limits of quantification were 0.12-10.52 ng/mL and 4.67-20.22 ng/mL, respectively. The recovery was 96.02-102.88% with a relative standard deviation of less than 3.02%. It is proposed that the methods described here can be applied for rapid evaluation, quality control and authenticity establishment of S. indica. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:125 / 139
页数:15
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