Fast method for DNA extraction in Nocardia and Saccharomonospora

被引：4

作者：

Salgado, A. ^{[1
]}

Ramirez, N. ^{[2
]}

Sandoval, E. ^{[1
]}

Sandoval, H. ^{[1
]}

机构：

[1] Univ Autonoma Metropolitana Xochimilco, Dept Sistemas Biol, Mexico City 04960, DF, Mexico

[2] Univ Autonoma Estado Mexico, Fac Med, Toluca 50180, Edo de Mexico, Mexico

来源：

JOURNAL DE MYCOLOGIE MEDICALE | 2008年 / 18卷 / 02期

关键词：

actinomycetes; microwaves; DNA isolation; halophiles;

D O I：

10.1016/j.mycmed.2008.04.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nowadays, the use of molecular techniques represents the most precise and fast methods in everyday research, this, perhaps, calls for the development of new methods to isolate DNA at low costs and with fast and reliable results. The DNA isolated using a microwave oven at different potencies and times, was of high purity and very suitable for later amplification procedures. The strains of microorganisms used were both actinomycetes and eubacteria, and in both cases it was tested on an halophile strain. The best isolations were obtained for Nocardia spp. at 400-480 W for 10-20 s, for Sacharomonospora spp. and Escherichia coli at 320-480 W for 15-20 s and for Salinicoccus spp. at 480-560 W for 10-20 s. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：100 / 102

页数：3

共 6 条

[1] Mycobacterium diversity and pyrene mineralization in petroleum-contaminated soils [J].

Cheung, PY ;

Kinkle, BK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (05) :2222-2229

[2] MICROWAVE TREATMENT OF SERUM FACILITATES DETECTION OF HEPATITIS-B VIRUS-DNA BY THE POLYMERASE CHAIN-REACTION - RESULTS OF A STUDY IN ANTI-HBE POSITIVE CHRONIC HEPATITIS-B [J].

COSTA, J ;

LOPEZLABRADOR, FX ;

SANCHEZTAPIAS, JM ;

MAS, A ;

VILELLA, A ;

OLMEDO, E ;

VIDAL, J ;

DEANTA, MTJ ;

RODES, J .

JOURNAL OF HEPATOLOGY, 1995, 22 (01) :35-42

[3] MICROWAVE-FIELD-DRIVEN ACOUSTIC MODES IN DNA [J].

EDWARDS, GS ;

DAVIS, CC ;

SAFFER, JD ;

SWICORD, ML .

BIOPHYSICAL JOURNAL, 1985, 47 (06) :799-807

[4]

PETER P, 2003, APPL ENVIRON MICROB, V69, P2153

[5] Dynamics of bacterial community composition and activity during a mesocosm diatom bloom [J].

Riemann, L ;

Steward, GF ;

Azam, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (02) :578-587

[6] GRAM-POSITIVE BACTERIA WITH A HIGH DNA G+C CONTENT ARE CHARACTERIZED BY A COMMON INSERTION WITHIN THEIR 23S RIBOSOMAL-RNA GENES [J].

ROLLER, C ;

LUDWIG, W ;

SCHLEIFER, KH .

JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :1167-1175

← 1 →