Elevated concentrations of interleukin-6 in intra-amniotic infection with Ureaplasma urealyticum in asymptomatic women during genetic amniocentesis

被引:38
作者
Bashiri, A
Horowitz, S
Huleihel, M
Hackmon, R
Dukler, D
Mazor, M
机构
[1] Ben Gurion Univ Negev, Fac Hlth Sci, Dept Microbiol & Immunol, IL-84105 Beer Sheva, Israel
[2] Soroka Univ, Med Ctr, Dept Gynecol & Obstet, Beer Sheva, Israel
[3] Soroka Univ, Med Ctr, Natl Ctr Mycoplasma, Beer Sheva, Israel
关键词
genetic amniocentesis; interleukin-6; intra-amniotic infection; Ureaplasma urealyticum;
D O I
10.1034/j.1600-0412.1999.780506.x
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Background. Intra-amniotic infection is an important cause of preterm delivery and interleukin-6 (IL-6) determination was recently applied for identification of microbial invasion of the amniotic cavity. Our aim was to determine the levels of IL-6 in culture-positive amniotic fluids at genetic amniocentesis and to evaluate their significance in relation to pregnancy outcome. Methods. Seven culture-positive and 23 culture-negative amniotic fluids, obtained at 17-19 weeks of gestation, were analyzed for IL-6 levels by an immunoassay (ELISA). Pregnancy outcomes of all 30 women were obtained from the medical charts. Results. The mean level of IL-6 in the culture-negative amniotic fluids was 78+/-206 pg/ml. Among the seven culture-positive, high levels of IL-6 were found only in three amniotic fluids that were culture-positive for Ureaplasma urealyticum (1834, 1342 and 2832 pg/ml). Low levels of IL-6, ranging from zero to 60 pg/ml, were found in four AFs that were culture-positive for Staphylococcus epidermidis (n=3) and Bacillus Gram-positive (n=1). Adverse pregnancy outcome occurred in the three women who had intra-amniotic infection with U. urealyticum accompanied by high levels of IL-6 (two fetal loss and one preterm delivery at 28 weeks of gestation). The four women with culture-positive but IL-6 negative amniotic fluids, had normal pregnancy outcome and term delivery. Two of the 23 women with culture-negative had preterm delivery, one with high (1000 pg/ml) and one with low (80 pg/ml) levels of IL-6. Conclusion. High levels of IL-6 are suggestive of a genuine intra-amniotic infection with ureaplasmas resulting in adverse pregnancy outcome, while culture-positive amniotic fluids with normal IL-6 levels, may suggest a state of contamination.
引用
收藏
页码:379 / 382
页数:4
相关论文
共 18 条
[1]   THE ACUTE-PHASE RESPONSE [J].
BAUMANN, H ;
GAULDIE, J .
IMMUNOLOGY TODAY, 1994, 15 (02) :74-80
[2]   Hypoxia stimulates cytokine production by villous explants from the human placenta [J].
Benyo, DF ;
Miles, TM ;
Conrad, KP .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (05) :1582-1588
[3]  
CASSELL GH, 1983, SEX TRANSM DIS, V10, P294
[4]  
Gomez R, 1997, PRETERM LABOR, P85
[5]   ADVERSE OUTCOME IN PREGNANCY FOLLOWING AMNIOTIC-FLUID ISOLATION OF UREAPLASMA-UREALYTICUM [J].
GRAY, DJ ;
ROBINSON, HB ;
MALONE, J ;
THOMSON, RB .
PRENATAL DIAGNOSIS, 1992, 12 (02) :111-117
[6]  
HOROWITZ S, 1995, J REPROD MED, V40, P375
[7]   ANTIBODIES TO UREAPLASMA-UREALYTICUM IN WOMEN WITH INTRAAMNIOTIC INFECTION AND ADVERSE PREGNANCY OUTCOME [J].
HOROWITZ, S ;
MAZOR, M ;
HOROWITZ, J ;
PORATH, A ;
GLEZERMAN, M .
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 1995, 74 (02) :132-136
[8]   UREAPLASMA-UREALYTICUM CERVICAL COLONIZATION AS A MARKER FOR PREGNANCY COMPLICATIONS [J].
HOROWITZ, S ;
HOROWITZ, J ;
MAZOR, M ;
PORATH, A ;
GLEZERMAN, M .
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS, 1995, 48 (01) :15-19
[9]  
HOROWITZ S, 1994, ISRAEL J MED SCI, V30, P450
[10]  
Kenny GE, 1980, MANUAL CLIN MICROBIO, P365