Centrin homologues in higher plants are prominently associated with the developing cell plate

被引:41
作者
DelVecchio, AJ
Harper, JDI
Vaughn, KC
Baron, AT
Salisbury, JL
Overall, RL
机构
[1] UNIV SYDNEY, SCH BIOL SCI A12, SYDNEY, NSW 2006, AUSTRALIA
[2] USDA, STONEVILLE, MS USA
[3] MAYO CLIN & MAYO FDN, DEPT BIOCHEM & MOL BIOL, ROCHESTER, MN 55905 USA
关键词
calmodulin; cell plate; centrin; confocal microscopy; immunochemistry; mitosis; plants;
D O I
10.1007/BF01279570
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Centrin and calmodulin are members of the EF-hand calcium-binding superfamily of proteins. In this study we compared localisation and immunoblotting of centrin with calmodulin in several monocot (onion and wheat) and dicot (mung bean and A. Arabidopsis) plants. We confirmed. that an anti-calmodulin antibody recognised a 17 kDa protein in all species tested and localises to the cytoplasm, mitotic matrix and with microtubules of the preprophase band and phragmoplast. In contrast, immunoblotting using anti-centrin antibodies shows that plant centrins vary from 17 to 20 kDa. Immunofluorescence microscopy with anti-centrin antibodies revealed only weak centrin immunoreactivity in the cytoplasm, nucleus, nuclear envelope, phragmoplast and mitotic matrix in meristematic cells. There was a slightly more intense perinuclear labelling in large differentiating onion cells and between separating anaphase chromosomes. While centrin is known to localise to the mitotic spindle poles in animal and algal cells, there was no appreciable immunoreactivity at the spindle poles in higher plants. In contrast, there was an intense immunofluorescence signal with anti-centrin antibodies in the developing cell plate. Further characterisation of the cell plate labelling by immunogold electron microscopy shows centrin immunoreactivity was closely associated with vesicles in the cell plate. Our observations suggest that centrin may play a role in cell plate formation.
引用
收藏
页码:224 / 234
页数:11
相关论文
共 61 条
[1]  
[Anonymous], CELLULAR BASIS GROWT
[2]   CENTRIN IS A COMPONENT OF THE PERICENTRIOLAR LATTICE [J].
BARON, AT ;
GREENWOOD, TM ;
BAZINET, CW ;
SALISBURY, JL .
BIOLOGY OF THE CELL, 1992, 76 (03) :383-388
[3]  
BARON AT, 1994, J CELL SCI, V107, P2293
[4]   ROOT MORPHOLOGY MUTANTS IN ARABIDOPSIS-THALIANA [J].
BASKIN, TI ;
BETZNER, AS ;
HOGGART, R ;
CORK, A ;
WILLIAMSON, RE .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1992, 19 (04) :427-437
[5]  
BASKIN TI, 1992, PLANTA, V187, P395
[6]   YEAST GENE REQUIRED FOR SPINDLE POLE BODY DUPLICATION - HOMOLOGY OF ITS PRODUCT WITH CA-2+-BINDING PROTEINS [J].
BAUM, P ;
FURLONG, C ;
BYERS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (15) :5512-5516
[7]   PROBING MICROTUBULE-ORGANIZING CENTERS WITH MPM-2 IN DIVIDING CELLS OF HIGHER-PLANTS USING IMMUNOFLUORESCENCE AND IMMUNOGOLD TECHNIQUES [J].
BINAROVA, P ;
RENNIE, P ;
FOWKE, L .
PROTOPLASMA, 1994, 180 (3-4) :106-117
[8]  
CHEVRIER V, 1992, J CELL SCI, V101, P823
[9]   MICROTUBULE NUCLEATING SITES IN HIGHER-PLANT CELLS IDENTIFIED BY AN AUTO-ANTIBODY AGAINST PERICENTRIOLAR MATERIAL [J].
CLAYTON, L ;
BLACK, CM ;
LLOYD, CW .
JOURNAL OF CELL BIOLOGY, 1985, 101 (01) :319-324
[10]   ORGANIZATION OF CORTICAL MICROTUBULES IN PLANT-CELLS [J].
CYR, RJ ;
PALEVITZ, BA .
CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (01) :65-71